Indonesia Conference Directory

Corresponding Author
AA Sg Weni Kumala Ratih

Institutions
a) Professional Clinical Psychology Master Students, Dept. Psychology, Airlangga University
b) Lecture and Clinical Psychologist, Dept. Psychology, Airlangga University

Abstract
Strategies for handling depression in divorced young adult has been scarely reported. Classical clinical evidences and international guidelines recommends psychotherapy management strategies, such as the cognitive behavioral approach, works well in handling and releasing from depressive conditions. The present case report aims to document and disseminate the treatment outcomes of the usefulness of Cognitive Behavioral Therapy for depression in reported case of a divorced young adult women. A brief clinical single case based report on the clinical manifestation, psychological assessment, therapeutic formulation and psychotherapeutic process on divorced young adult women (39 year old), diagnosed as DSM-V, with mild depression with somatic symptoms with onset usually occuring in young adulthood, referred to clinical psychologist for management. Based on a comprehensive psychiatry history taking, psychological assessment, and therapeutic formulation, Cognitive Behavioral Therapy for Depression (Miltenberger, 2008), was offered by professional psychology clinical master stundents guided by trained licensed clinical psychologist. Psychotherapy was given in 10 sessions, 60 minutes for each session within 5 weeks, and a follow-up of 3 monts. Symptoms were monitored for change by the therapist on 2 outcomes measures: Depression Anxiety Stress Scale and behavior observation during the process therapeutic. Clinical significant change (50% or above) based on pre- and post-therapy data scores on outcomes measures were used to assess the efficacy of the therapeutic intervention. Result indicate the intervention was effective for depression in a divorced young adult women. The challenges faced by the therapist in sensitive socio-cultural and limited client time, are discussed below.

Keywords
depression, divorce, young adult women, psychotherapy, cognitive behavioral therapy, case report

Topic
Health and Medicine

Link: https://ifory.id/abstract/qhrcVzDxkGv2

Corresponding Author
Anwar Ma ruf

Institutions
(a) Postgraduate School Universitas Airlangga
(b) Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga Surabaya

Abstract
The purpose of this study was to determine the molecular weight of the protein STAT STAT 5a and 5b that exist in liver tissue as a basis to determine the amino acid composition of broiler STAT protein phosphatase that is experiencing growth due to increased growth hormone (GH). Broiler liver tissue samples isolated from broilers were maintained for 21 days, then the examination followed by SDS Page and Western Blott. Results Western Blott known that STAT 5a protein molecular weight of 91 kDa with an amino acid composition ciggppkvmnmeesn or cysteine, isoleucine, glycine, glycine, proline, proline, lysine, valine, methionine, asparagine, methionine, glutamate, glutamate, serine, asparagines and STAT 5b of 90 kDa with an amino acid composition datnilvspvylypdip or aspartate, alanine, threonine, asparagine, isoleosine, leucine, valine, serine, proline, valine, tyrosine, leucine, tyrosine, proline, aspartate, isoleucine, proline.

Keywords
STAT 5a, STAT 5b, growth, broiler

Topic
Health and Medicine

Link: https://ifory.id/abstract/kPWdU3NrM6T8

Corresponding Author
I Gede Angga Adnyana

Institutions
Graduate student of Immunology, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia

Abstract
Background: Candidiasis si a fungal infection caused by Candida albicans. Synthetic drugs are often used in candidiasis treatment. Using synthetic drugs for a long period can leads to antifungal resistant phenomenon. Alternatif medicines from plants can be used as alternative to evercome this problem. Srikaya Leaves (Annona squamosa) are one of the sources of natural ingredients that have been shown to have various effects, including antifungals effect. Method: This study was aimed to determine the antifungal effects of srikaya leaf extract in inhibiting the growth of Candida in vitro. Srikaya leaf extract was obtained by maceration. After that, the extract was diluted into various concentrations (10% b/v, 20% b/v, 40% b/v, 80% b/v, and 100% b/v) with DMSO. Agar wells difusion method was chosen for testing the extracts antifungal effect. Tests were carried out 5 replications for each concentration. Results: Each concentration used in this study did not show any inhibition zone in the growth of Candida albicans in vitro, the same was found in negative controls. While positive control with Nystatin 20 mg showed average inhibition zone of 19 mm. Conclusion: Srikaya leaf extract has not been able to inhibit the growth of C. albicans in vitro. This can be caused by various factors, including the method of extraction and selection of fungal isolates. It is recommended to use a better extraction method and pure isolates of Candida albicans for further study.

Keywords
Candidiasis, Antimicrobial resistance, Annona squamosa, Fungal infection

Topic
Health and Medicine

Link: https://ifory.id/abstract/c9CpYaVkH6Dv

Corresponding Author
Ni Ketut Yuliana Sari

Institutions
(a) Postgraduate School Universitas Airlangga
Jalan Airlangga No. 4-6, Airlangga, Gubeng, Surabaya, Jawa Timur 60115, Indonesia
*ni.ketut.yuliana-2018[at]pasca.unair.ac.id

(b) Department of Biology, Faculty of Science, Udayana University, Bali
Jalan Kampus Bukit Jimbaran 80361 Bali, Indonesia

Abstract
Antimicrobial resistance has become a public health problem in the world, one of which is Methicillin Resistant Staphylococcus aureus (MRSA). One way to overcome this problem is to find new antibacterial compounds with the use of endophytic fungi. The purpose of this study was to test the antibacterial activity of crude extract of endophytic fungi Aspergillus sp. against MRSA and its contents in the secondary metabolites produced. Endophytic fungi Aspergillus sp. isolated from elephant ginger rhizome (Zingiber officinale var. Roscoe). This research is an exploratory research which is continued with experimental. The antagonist test of endophytic fungi Aspergillus sp. against MRSA produces a inhibition zone of 32 mm. The inhibitory of crude extract of endophytic fungi Aspergillus sp. in the water phase is 13 mm and an ethyl acetate phase is 9 mm. Phytochemical analysis of crude extract of endophytic fungi Aspergillus sp. in the water phase shows the presence of group compounds alkaloids, flavonoids, saponins, and terpenoids. GCMS analysis shows the content of glycidyl oleate (28,44%); didodecyl phthalate (26,67%); cis-13-Octadecenoic acid, methyl ester (20,73%); oleic acid, 3-hydroxypropyl ester (12,98%); 5Hydroxymethylfurfural (4,04%); benzophenone (2,42%); Propanamide, N-ethyl- (2,09%); benzene, nitro- (1,33%) and azulene (1,31%).

Keywords
Antibacterial; Endophytic fungi; Aspergillus sp.; Methicillin Resistant Staphylococcus aureus

Topic
Health and Medicine

Link: https://ifory.id/abstract/4H8mZUWNRArc

Corresponding Author
Aletheia Threskeia

Institutions
a) Post Graduate School, Universitas Airlangga, Surabaya, Indonesia
*aletheia.threskeia-2018[at]pasca.unair.ac.id
b) Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Abstract
Background: Enterotoxigenic Escherichia coli (ETEC) has been found as common cause of diarrhea in developing country. According to previous research by Jayakumari and friends in India, the leaf extract of Pisonia grandis has antimicrobial compounds. Therefore, researcher examines the use of Pisonia grandis leaf extract against ETEC. Objective: To determine whether the leaf extract of Pisonia gradis can be used as antimicrobial agent against Escherichia coli and ETEC. Methods: This was a true experimental research, which is done in the laboratory. The bacteria used is Escherichia coli and ETEC. Pisonia grandis leaves extracted with ethanol 70% is divided in eight different concentrations. Antimicrobial activity performed by broth dilution method to determine the Minimum Inhibitory Concentration (MIC). Results: There was turbidity changes at all test tubes up to fifty percent concentration , including the positive control tube, but there was no change in turbidity in the negative control tube. Growth of bacteria colonies was obtained when the tubes were planted in Nutrien agar. Conclusion: It can be concluded from the results that there was no antimicrobial activity in ethanol extract of Pisonia grandis leaves up to fifty percent concentration against Escherichia coli and ETEC.

Keywords
Pisonia grandis, Escherichia coli, Enterotoxigenic Escherichia coli, ethanol extract

Topic
Health and Medicine

Link: https://ifory.id/abstract/2kLm7fDuvVnJ

Corresponding Author
ni nyoman sumarsini

Institutions
a. Graduate Student of Immunology, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia
Jl. Airlangga No.4 - 6, Airlangga, Gubeng, Kota SBY, Jawa Timur 60115
b. Politeknik Kesehatan Kemenkes Mataram
Jl. Prabu Rangkasari, Dasan Cermen, Sandubaya, Kota Mataram, Nusa Tenggara Bar. 83232

Abstract
The infection is a condition the entry of microorganisms into the body multiply and cause disease. One of the causes of the infection is bacteria, antibacterial gift is one of the elect in dealing with infectious diseases. One of antibacterial from herbal plants, namely cananga flowers with the content of the active compounds that are able to act as an anti-microbial. The methods used to extract compounds rates cananga is extraction. Extraction techniques organic compounds used in this research is macerasion which is used polar methanol solvent. And in order to know the methanol extract compounds gaint the researcher used GCMS analysis. Then tested the sensitivity using methanol extract agains Gram positive and gram negative bacteria. So it is know the resistor power of methanol extract from cananga flowers (Canangium odoratum) against the growth of Gram positive and gram negative bacteria. This research uses observational analytical design using purposive sampling techniques. The results obtained are methanol extract in cananga flowers (Canangium odoratum) and the its resistor power against Gram Positive and gram negative bacteria as a role of antibacterical. The highest compounds in the extract is alpha-Bergamotene with area 22,65 % and the lowest compounds is Alpha, -Copaene and 9.12-Octadecadienoic acid (Z,Z)-, methyl Esther (CAS) Methyl linoleate with area 0.51 %. In addition, the extract is capable to inhibit the growth of Gram positive bacteria (Stapyloccocus aureus) with diameter of the 15 mm. Methanol extract of cananga flowers can only inhibit positive gram bacteria with diameter of the 15 mm.

Keywords
Cananga Flowers, Methanol Extract, Antibacterial Activity Test

Topic
Health and Medicine

Link: https://ifory.id/abstract/9TRZ3WgqLPzM

Corresponding Author
Pauzan Pauzan

Institutions
Immunology, Postgraduate Shool, Universitas Airlangga,

Abstract
Infectious diseases in Indonesia are still a major health problem. One of the bacteria that causes infectious diseases is Staphylococcus aureus. One of the traditional plants that can be used as an antimicrobial is red Meniran (Phyllanthus urinaria Linn) and red Betel (Piper crocatum), because it contains chemical compounds that act as antimicrobials such as flavonoids, alkaloids, saponins and tannins. The purpose of this study, to determine red Meniran extract, red Betel extract and a combination of both have antibacterial activity against Staphylococcus aureus ATCC 25923. Methods in this study using the method of diffusion. The concentrations used were 25% and 50% with a combination of 1: 1; 1: 2; 2: 1. The data from the research is then processed using statistical analysis of Analysis of Variance (ANOVA) with one-way method, to obtain the significance of the data. The results in the diffusion method obtained the greatest inhibitory effect of a single red Meniran extract at a concentration of 50% with an average of 26.7 mm inhibitory zone to Staphylococcus aureus ATCC 25923. The conclusion of this study is red Meniran has the most active antibacterial activity compared to red Betel and combinations.

Keywords
Meniran merah (Phyllanthus urinaria Linn), Red Betel (Piper crocatum), Staphylococcus aureus ATCC 25923 and antibacterial activity.

Topic
Health and Medicine

Link: https://ifory.id/abstract/BJyfRqPhUvLa

Corresponding Author
Elfred Rinaldo Kasimo

Institutions
Department of Health Analyst, Faculty of Health Science, Universitas Kadiri, Kediri, East Java, Indonesia

Abstract
Soil Transmitted Helminths (STH) is a worm of the intestinal nematode that is transmitted to humans through soil contaminated with feces. In STH infection, CD4 cells differentiate four types of T-helper cells, namely Th1, Th2, Th17, and Treg. Th2 cells secrete cytokines in the form of IL-4, IL-5, IL-9, and IL-13 which activate various mucosal epithelial cells, eosinophils, basophils, IgE production, mast cells, and goblet hyperplasia cells. Basophil cells and mast cells activated by IgE through crosslinked-high-affinity Fc receptors (FcRs) from IgE on the cell surface cause secretion from inflammatory mediators in the form of TNF-α, TGF-β, IL-1, IL-6, and mediators performed in the form of histamine and heparin which increase smooth-muscle-cell motility, so that it stimulates entry into the intestine and increases secretion by goblet-cells which will expose the worms out of the body. This type of research uses a case control. The total subjects were 40 farmers, consisting of 20 people infected with STH and 20 people not infected with STH in Sumberagung Hamlet, Gurah Sub-District and Janti Hamlet, Papar District, Kediri Regency. The results of this study showed no difference in the number of basophils in farmers infected and not infected with STH with a value of p=0.418 or p>0.05. There are differences in TNF-α levels in farmers infected and not infected with STH with a value of p=0.019 or p<0.05. TNF-α levels in farmers infected with STH are lower than those that are not infected because infections occur repeatedly and continuously that form immunotolerant

Keywords
soil transmitted helminth, basophils, tumor necrosis factor-α

Topic
Health and Medicine

Link: https://ifory.id/abstract/tmbT8nZzrPdw

Corresponding Author
Intashofal Laili

Institutions
Postgraduate School, Universitas Airlangga
intashofal.laili-2018[at]pasca.unair.ac.id

Abstract
Adherence is a condition where the patient adheres to his treatment on the basis of his own consciousness, not merely obeying the doctors orders. Compliance is an important factor in the success of ARV therapy. This research uses the design of Mann Whitney test. The variables in this study consisted of dependent variables namely medication adherence and independent variables of CD4 increase in HIV AIDS. The population in this study were people with HIV AIDS in Syarifah Ambami Hospital Rato Ebu Bangkalan. Samples were taken by PLHIV who conducted CD4 examinations and within the same time period with a total sample of 61 people. Data collection using a questionnaire. Data is analyzed using the Mann Whitney test and the results showed that adherence to taking medication for CD4 levels was obtained a P value = 0,000 (α: 0.05). This shows that there are differences in CD4 that are obedient and non-adherent taking medication in people with HIV AIDS in Syarifah Ambami Hospital Rato Ebu Bangkalan. Adherence to taking antiretroviral drugs is needed in people with HIV AIDS to increase self-esteem, through involvement in peer support activities.

Keywords
Drug compliance, CD4

Topic
Health and Medicine

Link: https://ifory.id/abstract/LnWHkKX2UAPN

Corresponding Author
Intashofal Laili

Institutions
Postgraduate School, Universitas Airlangga
intashofal.laili-2018[at]pasca.unair.ac.id

Abstract
Adherence is a condition where the patient adheres to his treatment on the basis of his own consciousness, not merely obeying the doctors orders. Compliance is an important factor in the success of ARV therapy. This research uses the design of Mann Whitney test. The variables in this study consisted of dependent variables namely medication adherence and independent variables of CD4 increase in HIV AIDS. The population in this study were people with HIV AIDS in Syarifah Ambami Hospital Rato Ebu Bangkalan. Samples were taken by PLHIV who conducted CD4 examinations and within the same time period with a total sample of 61 people. Data collection using a questionnaire. Data is analyzed using the Mann Whitney test and the results showed that adherence to taking medication for CD4 levels was obtained a P value = 0,000 (α: 0.05). This shows that there are differences in CD4 that are obedient and non-adherent taking medication in people with HIV AIDS in Syarifah Ambami Hospital Rato Ebu Bangkalan. Adherence to taking antiretroviral drugs is needed in people with HIV AIDS to increase self-esteem, through involvement in peer support activities.

Keywords
Drug compliance, CD4

Topic
Health and Medicine

Link: https://ifory.id/abstract/aqbPdypmYL3G

Corresponding Author
Rian Anggia Destiawan

Institutions
a) Post Graduate School, Universitas Airlangga, Surabaya, Indonesia
*rian.anggia.destiawan-2018[at]pasca.unair.ac.id
b) Veterinary Faculty, Universitas Brawijaya, Malang, Indonesia

Abstract
Egg is raw product that can not long lasting. It was contaminated easily because they had pores and source of microorganism nutrients. The room temperature that was high can made the egg survived just for 14 days, to maintain the quality and safety of egg, egg processing was needed, it was coated the eggs with natural ingredients with antimicrobial agent like chitosan. In this study, Chitosan was obtained from deacetylation of chitin from shrimp shell waste. Then, chitosan was tested with antimicrobial effectiveness. After being tested, the eggs were dipped into chitosan with different time, they were at 10, 20 and 30 seconds. Furthermore, the eggs were stored in room temperature with storage time of 0, 7, 14, 21, 28, and 35 days. It was examined with high of air pocket, yolk index, albumin index, HU result, and microbiological examination test of the eggs (TPC, Salmonella sp, and fungal). In this study, the best result of egg quality was at 30 seconds of room temperature storage.

Keywords
Egg; Chitosan

Topic
Health and Medicine

Link: https://ifory.id/abstract/dTzNkAYywG8r

Corresponding Author
Anggi Ardilla

Institutions
a) Postgraduate School, Universitas Airlangga, Jl. Airlangga No.4-6, Surabaya, Indonesia
b) Faculty of Nursing and Health University of Muhammadiyah Semarang

Abstract
Anticoagulants are commonly used in hematologic laboratories to thin the blood, anticoagulants normally used by EDTA, Garlic also has a compound that serves as an anticoagulant, but not all anticoagulants can be used because it can affect the morphology of blood cells. The purpose of this study was to compare the erythrocyte morphology using EDTA anticoagulants and garlic filtrates as alternative anticoagulants. This type of research is analytic. The sample was taken by non-random purposive sampling as many as 33 students from the total population of 50 students of semester VIII D IV of Health Analyst University of Muhammadiyah Semarang, then identified the erythrocyte morphology on EDTA anticoagulant samples and garlic filtrate. The result of the examination showed that there were 2 samples of samples using EDTA anticoagulant. There were 2 samples of crenation, while those using garlic 1 filtrate were crenated, this showed that samples using EDTA anticoagulant were slightly more likely to occur than garlic filtrate. Chi square statistic test obtained value of significance of 0.061 with significance level of 0.05 that is> 0.05 so it can be concluded that there is no difference of erythrocyte morphology using EDTA anticoagulant and garlic filtrat as alternative anticoagulant.

Keywords
EDTA anticoagulant, garlic filtrate, erythrocyte morphology

Topic
Health and Medicine

Link: https://ifory.id/abstract/uvEWMpBPRUjL

Corresponding Author
Yohana Siera Riani Rozali

Institutions
1) Department of Immunology Postgraduate School, Airlangga University, Surabaya, Jawa Timur, Indonesia
2) Department of Clinical Pathology, Faculty of Medicine Airlangga University, Dr. Soeotomo Hospital Surabaya, Jawa Timur, Indonesia
3) Department of Parasitology, Faculty of Medicine Airlangga University, Surabaya, Jawa Timur, Indonesia

Abstract
Human immunodeficiency virus (HIV) is a virus that attacks immune system, namely CD4 T cells. T cells play an important role in the regulation of the immune system both against pathogens such as viruses, bacteria, and parasites. HIV sufferers will show a decrease in endurance characterized by a decrease in T cell function due to T cell death. This results in tuberculosis easily occurring in people with HIV. Interleukin 10 (IL-10) is one of the anti-inflammatory cytokines that can affect the immune system. IL-10 can increase T cell proliferation, activation of B cells and induction of specific genes in the process of phagycosis. The purpose of this study was to determine the association of CD4 T-cell and IL-10 deaths in HIV-infected patients with secondary co-infection with pulmonary tuberculosis. This study was an observational analytic study with a cross-sectional method from October 2018 to January 2019 at UPIPI Hospital Dr. Soetomo Surabaya. The research subjects were 34 patients who were selected based on the GenExpert results and those who had at least 6 months of antiretroviral therapy. Examination of cell death was carried out by flowcytometry and IL-10 method using the ELISA method. Analysis of the correlation of cell death and IL-10 using Spearman. Test the normality of data using Shapiro-Wilk. The average number of cell deaths (%) and IL-10 (pg / ml) levels in the study subjects was 92.97 ± 23.63 and 3.20 ± 1.09 with the correlation test analysis being 0.200. The results of the analysis of cell death and IL-10 levels did not have a significant difference.

Keywords
Cell death, CD4 T-cell, Interleukin-10. HIV, Tuberculosis

Topic
Health and Medicine

Link: https://ifory.id/abstract/wGL8xvb76qBQ

Corresponding Author
Ni Nyoman Ariwidiani

Institutions
a) Postgraduate School, Universitas Airlangga, Jl. Airlangga No.4-6, Surabaya, Indonesia
*ni.nyoman.ariwidiani-2018[at]pasca.unair.ac.id
b) Health Analysts Department, Polytechnic Health Ministry of Mataram, Indonesia

Abstract
Malaria is an infectious disease, which attacks the red blood cells and causing a decrease of hemoglobin levels and showing typical symptoms of liver disorders such as hepatomegaly and splenomegaly. However, quantitatively correlations between the type and parasitemic number with hemoglobin and protein levels are still not clearly known. the purpose of this study to determine the correlation between type and parasitemia numbers with hemoglobin and total proteins levels in malaria patients. this study was an observational analytic study with a case-control design. the subject of this study taken by accidental sampling. the data showed the most type of parasite in the research areas are Malaria falciparum and Malaria vivax with parasitemia numbers 1 to 4. the mean of hemoglobin levels for Malaria falciparum is 11.1 g/dl and Malaria vivax is 10.4 g/dl. meanwhile, the proteins levels for Malaria falciparum is 6.3 g/100 ml and Malaria vivax is 6.2 g/100 ml. The correlations with statistical test showed no decrease in hemoglobin and total protein levels with probability is 0.102 (p <α = 0.05). the conclusion of this study is there no correlation between type and parasitemia numbers with hemoglobin and total proteins levels in malaria patients.

Keywords
Hemoglobin, type of malaria, parasitemia numbers, totals protein levels

Topic
Health and Medicine

Link: https://ifory.id/abstract/WUyEmeqvrb8z

Corresponding Author
Jessika Virgiana

Institutions
(1)Department of Immunology, Postgraduate School, Universitas Airlangga, Surabaya, East Java, Indonesia
(2)Department of Clinical Pathology, RSUD Dr. Soetomo, Surabaya, East Java, Indonesia
(3)Department of Public Health and Preventive Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
(4)Department of Health Analyst, Faculty of Health Science, Universitas Kadiri, Kediri, East Java, Indonesia

Abstract
One of the diseases whose prevalence continues to increase and needs serious attention in the GBD is Acquired Immune Deficiency Syndrome (AIDS). The number of new cases of HIV in 2014-2016 was 104,896, the number of cases was quite large in the province of East Java with 15,176 people. Patients with AIDS show damaged CD8+ T cells (CTL), even though HIV does not infect CD8+ T cells. This is because CD4+ T cells (the main target of HIV) are needed for the response of CD8+ T cells to antigens. HIV infection reduces the number of CD4+ T cells and cytokines released by T lymphocytes, such as interferon-γ (IFN-γ) and interleukin-2 (IL-2). After an HIV positive patient is exposed to Mycobacterium tuberculosis, IFN-γ decreases, reducing the response of helper 1 T cells (TH1). This study is an observational study with a cross sectional study design that is analytical. Representative data was performed on whole blood and analyzed on a dual-laser BDFACS brand flow cytometer with laser excitation at 488 nm and 635 nm. The results of this study are that there is no significant relationship between CD8+ count and IFN-γ in HIV-infected patients with pulmonary tuberculosis infection at UPIPI Dr. Soetomo Hospital Surabaya. The greater the count of CD8+ respondents, the higher the IFN-γ level will tend to be.

Keywords
CD8+ T cell, IFN-γ level, HIV/AIDS patient

Topic
Health and Medicine

Link: https://ifory.id/abstract/G4eU8WDA7xay

Corresponding Author
Maulida Julia Saputri

Institutions
(a) Postgraduate School, Universitas Airlangga
Jl. Airlangga No.4-6, Surabaya, Indonesia
(b) Lecturer in Health Analyst Faculty of Nursing and Health, Universitas Muhammadiyah Semarang
*ana_hidayati[at]unimus.ac.id
(c) Lecturer in Chemistry Education Faculty of Mathematics and Natural Sciences, Universitas Muhammadiyah Semarang

Abstract
Research has been carried out on reducing CO gas levels using ZSM-5 membranes synthesized at low temperatures with various treatments in the gauze 304-200 mesh. The purpose of these various treatments is to make ZSM-5 zeolite precursors grow very well on the surface of the gauze. Variations of the 4 treatments used were soaked in HNO3 10% at 60ºC and acetone; soaked in toluene 95% and HCl 15%; soaked in NaOH 20%, HCl 15% and electroxidated with H2SO4 20%; and soaked in toluene 95%, HCl 15%, and TPABr 0.1 M. This study is an experimental study supported by the literature. the CO gas used source of cigarettes burned for 5 minutes in a closed reactor chamber with a volume of 18 liters through the ZSM-5 membrane for 10 minutes. Reduction of CO gas with 4 treatments is 9.42%; 6.36%; 10.26% and 7.75%. The largest percentage reduction of CO gas in the third treatment was soaked in NaOH 15%, HCl 15% and electroxidated with H2SO4 20%.

Keywords
Membrane ZSM-5, CO gas concentration, treatment variation gauze, gauze 304- 200 mesh.

Topic
Health and Medicine

Link: https://ifory.id/abstract/PEK4Ct6Uh2QT

Corresponding Author
Anggraeni Puspitasari

Institutions
a) Forensic Science Program, Post Graduate Studies, Universitas Airlangga, Surabaya, Indonesia
*anggraeni.puspitasari-2018[at]pasca.unair.ac.id
b) Poltekes Kemenkes Jambi, Indonesia

Abstract
Red dragon fruit (Hylocereus polyrhizus) is one of cactus plant group containing iron, vitamin B1, Vitamin B2, vitamin B3, Vitamin C, and others. Vitamin C is a water-soluble vitamin can be antioxidants. People commonly store red dragon fruit because its considerable size and does not consume a single meal, however length storage can decrease nutritional value of the fruits because vitamin C is easily oxidized . The aim of this study was to determine effects of length storage toward levels of vitamin C in red dragon fruit during 1 until 3 days. The study was experimental design through cross sectional approach. 3 samples were taken by random sampling were purchased at Pasar Buah Kota Jambi. Vitamin C levels used Iodimetry method were conducted at the Chemistry Laboratory of the Academy of Health of Jambi Province. The results showed that average vitamin C level in red dragon fruit in 1 day storage was 12,1405 mg, 2 days storage was 11,0369 mg and 3 days storage was 6,9860 mg. Statistical test used the ANOVA test concluded that there was an effect of vitamin C levels on dragon fruit stored for 1 day, 2 days and 3 days.

Keywords
Red Dragon Fruit, Length Storage, Vitamin C

Topic
Health and Medicine

Link: https://ifory.id/abstract/dNa8repqKuZ6

Corresponding Author
Anas Fadli Wijaya

Institutions
Graduate Student of Immunology, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia
Medical Laboratory Techniques,Polytechnic of Health, Mataram, Indonesia

Abstract
Fungi are heterotrophic organisms which require organic compound as their source of nutrition. Based on the cell type, fungi are classified into unicellular (yeast) and multicellular (mold). One of standard laboratory examination for fungi is microbiological culture using PDA (Potato Dextrose Agar) media. The addition of sucrose in the PDA is to enrich the media composition and the energy source that will aid the growth process of mold and yeast. This study aimed to determine the effect of sucrose addition in PDA media to the growth of mold and yeast using quasi-experimental design. Sample of mold and yeast used in this research were Rhizopus sp and Candida albicans, respectively. Candida albicans isolates were made into suspension adjusted to be equivalent with the turbidity of 0,5 Mac Farland and diluted 106 times. Growth diameter of Rhizopus sp was measured and the colony number of Candida albicans were counted on PDA supplemented with 1 g, 2g, and 3g, respectively. The results were compared to those of control group and analyzed quantitatively. One Way Anova test showed p value of 0.000 (p<α) on Rhizopus sp and Kruskal Wallis test showed p value of 0.009 (p>α) on Candida albicans, suggesting a significant effect of sucrose addition to the growth of mold but no effect to the growth of yeast.

Keywords
Sucrose, PDA media, Candida albicans, Rhizopus sp, Mold, Yeast

Topic
Health and Medicine

Link: https://ifory.id/abstract/DaYvuxtkrz37

Corresponding Author
Htet Htet Aung

Institutions
a)Forensic Science Program
Postgraduate School
Airlangga University
4-6 Airlangga Rd., 60286 Surabaya – Indonesia.
*htethtetaung1994mya[at]gmail.com
b)Forensic Science Program
Postgraduate School
Airlangga University, 4-6 Airlangga Rd., 60286 Surabaya – Indonesia.

Abstract
The presence of spermatozoa in the vagina is a sure sign of intercourse. In some cases microscopic examination give no evidence or establish false negative results. This may either be by absence of ejaculate, oligo/azoospermia, vasectomy, sperm degeneration or sampling and storage factors. Under such condition, other ejaculate components, namely the enzyme acid phosphatase, choline has to be thought. To establish the effectiveness of such ejaculate components/test, this study through direct and indirect method examined 192 clothes semen patches by phosphatase acid and zinc test on day 1, 7 and 3 months. On the first method, washing was done then examined; whereas the second method had simultaneous washing and tests with 7 different brands of detergents on 4 different semen patches and water as a control. The results showed that the sensitivity was very low (0.186) while the specificity was very high (100%). This shows that both tests have high specificity values: phosphatase test for the presence of phosphatase enzyme, zinc test for zinc in semen. Despite of the specificity, phosphohydrolase-phosphatase enzyme is though susceptible to degradation by temperature, humidity and chemical substance of element SDS (Sodium Dodecyl Sulfate) which has the ability to cut enzymes.

Keywords
acid phosphatase test, effectiveness, identification, intercourse, semen spots, zinc test

Topic
Health and Medicine

Link: https://ifory.id/abstract/LQ4ZndTjeMkG

Corresponding Author
Suryani Dyah Astuti

Institutions
Universitas Airlangga

Abstract
This study aims to determine the blue laser diode irradiation angle and the optimum time for bacterial photoinactivation. Staphylococcus aureus. The research was carried out at various angles and lengths of irradiation time. Data analysis using anova factorial to determine the effect of each factor. The results showed the influence of blue diode laser irradiation angle on inactivation of Staphylococcus aureus bacteria. Laser diode 403 nm exposure with an angle of 90 lurus perpendicular to the surface of the absorbent medium and exposure time (90 ± 0.005) is optimal for reducing bacterial viability with a percentage decrease in bacterial viability of (72.7814 ± 4.1361)% not significantly different from the 80o angle. So the angle of exposure and duration of irradiation has an effect on the photodynamic effectiveness of bacterial inactivation.

Keywords
irradiation angle, energy density, laser diode, inactivation, Staphylococcus aureus

Topic
Health and Medicine

Link: https://ifory.id/abstract/MdYrpV69ukXC

Corresponding Author
Diah Indriani

Institutions
Biostatistics and Population Studies Department, Faculty of Public Health, Universitas Airlangga

Abstract
The Continuum of Care concept aims to improve health status starting from pre-pregnancy, pregnancy, maternity, postpartum, infants, toddlers, children, adolescents, adults and the elderly. The recording and reporting of health services is very necessary in the implementation of the continuum of care. In Indonesia, medical records have not been well integrated. Only in certain parts has it begun to be integrated, for example ANC record for mother, Posbindu records and records of the Elderly Posyandu. UKS is one of the potential school unit for the continuity of student health records. The Ministry of Health has published a Health Record Book for students at school, but the implementation of the book is still not optimal. They also develop dokter kecil program for student. Dokter kecil is student who get responsibilty as a role in their health attitude. This study aims to develop a UKS empowerment system along with a dokter kecil as a center for recording the health status of students in elementary schools. This research is a qualitative research by conducting in-depth interviews with principals, UKS teachers and conducting training for dokter kecil about student health notebooks. The variables observed were activities in UKS related to health records, dokter kecil activities and the role of UKS teachers, UKS infrastructure and school commitments. The results of the study indicate that UKS can be used as a training center for dokter kecil to learn to record student health status. Dokter kecil are given training to understand basic anthropometry and record it in the student health notebook. The results of observations conducted every month indicate that the level of skills of the dokter kecil in making basic anthropometric measurements and calculating the nutritional status of his friend was getting better. If a comparative analysis is carried out on 38 students there are differences in skill progress with p <0.05. Dokter kecil and UKS can mobilize activities to record student health status. Dokter kecil of students in grades 4, 5 and 6 can easily understand and do the task well.

Keywords
dokter kecil, UKS, student health status recording

Topic
Health and Medicine

Link: https://ifory.id/abstract/Lz9QaBXKW7GU

Corresponding Author
I Gusti Agung Putu Mahendrayasa

Institutions
Fakultas Kesehatan Masyarakat Universitas Airlangga

Abstract
Japanese Encephalitis is one of the main causes of inflammation in the human brain. This case most commonly found in the Province of Bali. Positive case discoveries experienced an increased from 2014 to 2015 and decreased in 2016 and 2017. The Japanese Encephalitis disease prevention and control program has not been carried out optimally, resulting in case of “under reported”. The purpose of this research is to evaluate the implementation of the Japanese Encephalitis surveillance system based on surveillance attributes at the Bali Provincial Health Office. This research used descriptive study with design of “a cross-sectional research”. The research informants numbered 10 people which consisted of surveillance officers at the District or City and Bali Province Health Office. The research variables that used in this research are Inputs, Process, Output and Surveillance Attributes. The results of this study indicate that the most “positive cases” of Japanese Encephalitis in Bali Province mostly occurs in the group of male aged 1-4 years, in Buleleng Regency on March 2017. The problems with Input are data sources and the absence of risk factor data. The problem with the process are data analysis and interpretation have not been carried out routinely. The problems with Output are epidemiological information, information dissemination and feedback have not been routinely implemented. The surveillance attribute showed the Japanese Encephalitis surveillance system are not simple, inflexible, low acceptability, not sensitive, low positive predictive value and low data quality. The conclusion is the surveillance system has not been effective and efficient.

Keywords
Japanese Encephalitis, Evaluation, Surveillance System, Atribute

Topic
Health and Medicine

Link: https://ifory.id/abstract/Rtn8JucpDZBE

Corresponding Author
Muchammad Yunus

Institutions
Faculty of Veterinary Medicine, Airlangga University

Abstract
This study was to know induction of protective immunity that represented endogenous development of parasites and oocyst production using some low doses of E. tenella oocysts through challenged test. The present study used 55 broiler chickens at 3 weeks old were divided into 4 groups. Group 1 was 10 chickens administered aquades, after 14 days administered aquades then challenged 5 x 103 E. tenella oocysts, 4 days post challenge 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination, while oocysts production was calculated on 5 other chickens from day 6 to 12 post challenge. Group 2, 3 and 4 which each group consisted 15 chickens inoculated 1 x 102, 2 x 102, 3 x 103 E. tenella oocysts respectively, 4 days pi 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination, while oocysts production was calculated on 5 other chickens from day 6 to 12 post for primary infection. Fourteen days after primary infection, each group (2, 3 and 4) were challenged 5 x 103 E. tenella oocysts, 4 days post challenge 5 chickens were sacrificed to observe morphological endogenous development of parasites by histopathological changes examination, while oocysts production was calculated on 5 other chickens from day 6 to 12 post challenge. The results showed that group 1, in challenged test, parasites were development and proliferation well so that oocyst production clearly increased, whereas group 2, 3 and 4 were seen few development and proliferation of parasites, there were no significant oocysts production around group. In conclusion, the endogenous development disabilities of parasites occur as a result of protective immunity generated resulting from the first antigen exposure so that proliferation and multiplication of parasites became decreased.

Keywords
E. tenella, low doses, protective immunity

Topic
Health and Medicine

Link: https://ifory.id/abstract/qkArwVb8ZBXf

Corresponding Author
Nove Hidajati

Institutions
(a) Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga Surabaya
(b) Department of Anatomi Veterinary, Faculty of Veterinary Medicine, Universitas Airlangga Surabaya

Abstract
Ghrelin is a gastric peptide that plays an important role in regulating food that enters the body (food intake). Before eating plasma ghrelin concentration rises gradually and immediately drops after eating. Addition of ghrelin intravenously increases food intake and triggers appetite (appetite), this proves that ghrelin plays a role in hunger and the initial desire to eat (meal initiation). Ghrelin was also involved in weight control because the body mass index was negatively controlled by plasma ghrelin concentration at fasting time. Signal abnormalities originating from the stomach will be related to energy balance disorders, growth and this is related to gastrointestinal and neuroendocrine function. Knowledge that shows between the relationship of ghrelin, ghrelin receptors, hypothalamus and the implications of ghrelin in controlling gastrointestinal function, energy balance, current growth is still not entirely clear. In previous studies it was known that in broiler chickens the molecular weight of ghrelin protein is 13.4 kDa and the amino acid composition of the ghrelin protein is mflrvil or methionine, phenylalanine, leucine, arginine, valine, isoleucine, leucine. The results showed that the exploration of natural ingredients came from Ipomoea aquaticum, Nasturtium officinale, Manihot esculena showed the presence of ghrelin protein. Ghrelin results from plants are then used for experiments in several experimental animals. With the discovery of ghrelin protein from natural ingredients from plants, it can be used to regulate energy balance so that there is an increase in feed efficiency.

Keywords
Ghrelin, Plant Ghrelin, Energy Balance, Efficiency Feed

Topic
Health and Medicine

Link: https://ifory.id/abstract/rMRn62HfLABD

Corresponding Author
Prihartini Widiyanti

Institutions
(a) Department of Physics, Faculty of Science and Technology Universitas Airlangga

Abstract
Zinc oxide and eugenol (ZOE) dental cements are widely used in dental restorations. This cement has low heat transfer properties, protects the pulp from phosphoric acid of phosphate cement or silicates and does not irritate the pulp. But ZOE has lowest mechanical properties and high solubility properties. From these shortcomings, it is necessary to change the size of zinc oxide material. The size of the micrometer is changed to nanoparticles in order to accelerate the formation of cement and increase the strength of cement. The sample used in this in vivo study was made from zinc oxide and eugenol dental cement and zinc oxide nanoparticles. Nano cement of this tooth has a composition of zinc oxide of 0.4 g, 0.45 g, 0.5 g and 0.55 g and eugenol 0.2 ml liquid. The experimental animals used in this study were Riverine rabbit species (Bunolagus monticularis). The interaction time of nano zinc oxide dental cement in rabbit teeth are 7, 14, 21 and 30 days. Based on the results of compressive strength test, it is obtained that the compressive value is higher in the composition of nano zinc oxide eugenol 0.55 g. Furthermore, along with the longer duration of interaction time between nano zinc oxide eugenol dental cement in rabbit teeth the better result obtained. This is supported by the microstructure of the sample captured using Scanning Electron Microscopy (SEM). SEM result is indicated disguised connection between the zinc oxide eugenol dental cement and rabbit teeth.

Keywords
microstructure, zinc oxide nanoparticles, zinc oxide and eugenol cement

Topic
Health and Medicine

Link: https://ifory.id/abstract/NDvFbweXBCy6

Corresponding Author
Fery Setiawan drg

Institutions
a) Postgraduate Student Universitas Airlangga
Jalan Airlangga 4-6, Surabaya 60285, Indonesia
b) Oral Biology Department Faculty of Dentistry Universitas Airlangga
Jalan Prof. Dr. Moestopo 47, Surabaya 60132, Indonesia
*jenny-s[at]fkg.unair.ac.id
c) Graduate Student Faculty of Dentistry Universitas Airlangga
Jalan Mayjend Prof Dr Moestopo 47, Surabaya 60132, Indonesia

Abstract
Wound healing is still remaining a problem worldwide, especially in dentistry. Wound healing has four stages , that in this article will be focused on the inflammatory phase. TNF-alpha is cytokine related with wound healing and one of its receptor called Tumor Necrosis Factor Receptor 1 (TNFR1) related with TrkA and NF-KB. The former relates with peptidergic nociceptors (Substance P (SP), Calcitonine Gene Related Protein (CGRP)) and the latter relates transcription factor. Capsaicin is one of the traditional herbals that has vasodilatation effect, so that it can enlarge the blood vessels and shorten the duration of wound healing. This study was analytic experimental laboratory. Post weaned wistar rats (rattus norvegicus) were divided into three groups, K0 negative control group, K1 positive control group, and K2 treatment group. Both K1 and K2 were administered with Aggregatibacteractinomycetemcommitans serotype b on the surface of gingiva, and K2 was alse administered with extract cayenne pepper with dose 0.0912 mg/kg/day. A week after the treatment, the rats were sacrificed with thionembutal and then it was done HE staining and immunohistochemistry method using polyclonal antibody on TNF-Alpha and TRPV-1 expression. Kolmogorov-smirnov and posthoc test showed that TRPV-1 count and TNF-Alpha expression of the K2 to be significantly higher than the K0 and K1 groups (p<0.05), while there was no significant difference in the TRPV-1 and TNF-Alpha expression between K0 and K1 groups(p>0.05). Capsaicin promotes the number of TNF-Alpha so that it can be confirmed as the alternative treatment of periodontitis.

Keywords
Capsaicin, TRPV-1, TNF-Alpha, TNFR1, TrkA, NF-KB

Topic
Health and Medicine

Link: https://ifory.id/abstract/MZuCqpTN8x6R

Corresponding Author
Ika Nur Pratiwi

Institutions
1Faculty of Nursing Universitas Airlangga, Kampus C UNAIR, Mulyorejo, Surabaya, 60115, Indonesia.
*ikanurpratiwi[at]fkp.unair.ac.id

Abstract
Rabies is an important animal-borne disease in Indonesia with potentially fatal outcome. Initial management following animal bite may alter prognosis of the disease. This study aims to evaluate the effect of an integrated lecture and discussion (ILD) on mothers- knowledge and attitude toward initial management of rabies related to dog bite. This is a quasi-experimental study with pre-and post-test design conducted at district Merti Jaya, West Kalimantan Province. Two groups were formed; intervention group who received ILD related to rabies initial management, and control group who were given leaflet of the disease. The outcomes measured were knowledge and behavior of mother at the end of study. There were 72 participants with equal number in both groups. Age, education and employment status were similar between the groups. There was a significant increase on mother-s knowledge in intervention group after ILD (Meanstandard deviation, Pretest 12.31.7, Posttest 14.00.9, P < 0.05). This was also seen in control group (Pretest 11.61.9, Posttest 12.81.4, P < 0.05). However, significant improvement was not seen in mother-s attitude (all P value > 0.05). Integrated lecture and discussion may improve mothers- knowledge pertaining rabies initial management. Further development in information delivery need to be done to alter mothers- attitude toward rabies

Keywords
integrated lecture and discussion, mother-s behavior, dog bite, rabies

Topic
Health and Medicine

Link: https://ifory.id/abstract/z9dH3Qhpbcwk

Corresponding Author
Anak Agung Istri Dalem Cinthya Riris

Institutions
a* Postgraduate School, Universitas Airlangga, Jalan Mayjen Prof. Dr. Moestopo No.47, Surabaya, Indonesia
*a.a.istri.dalem.cinthya-2017[at]fk.unair.ac.id
b Undergraduate School, Universitas Airlangga, Jalan Mayjen Prof. Dr. Moestopo No.47, Surabaya, Indonesia

Abstract
Madegraft is a new method which is adopted from universal preconception method and was made to simplify the application in Indonesia. This method helps women to increase self-efficacy and evaluate their own cycle, then they can prepare for a conception. This study aims to describe about a midwife-s perspectives through madegraft application. Single-case single-side case design was selected as the method of this study and another midwife was involved to take out the observation. The data were collected through observations, interviews, and document analyses. Our findings suggest that the preconception care using madegraft was performed in three stages: (1) menstrual cycle, (2) peak day, and (3) fertility booster. Our study confirms that the madegraft can be a tool to help a midwife become a role model for preparing conception care.

Keywords
madegraft; method; midwife; conception

Topic
Health and Medicine

Link: https://ifory.id/abstract/mvLW23T4dMyA

Corresponding Author
Nunuk Dyah Retno Lastuti

Institutions
Universitas Airlangga

Abstract
ABSTRACT In Indonesia, genetic research for Sarcoptes scabiei in rabbits is still limited. The development of diagnostic kits and sub-unit vaccines for the prevention of scabies is urgently needed because several studies state that S. scabiei is likely to be resistant to anti-scabies drugs such as ivermectine. Early studies carried out genetic identification of S.scabiei mites from scabies infected rabbit from several rabbit farms in East Java. The purpose of this research was to identify the COX-1 gene from S. scabiei-s mitochondrial DNA, isolated rabbits from Pacet and Lawang, East Java, and used specific primers with target of 290 bp. The research methods were as follows: isolation and identification of S. scabiei from rabbits infected with scabies, DNA extraction with QIAamp DNA mini kit (Qiagen, Hilden, Germany), amplification by PCR. PCR products from S. scabiei isolates from Pacet and Lawang rabbits produced bands of around 290bp with 2% agarose gel electrophoresis. The research still needs to be continued for the analysis of sequencing of the COX-1 S. scabiei gene to identify the homology between rabbits from various regions with GenBank data.

Keywords
East Java, Genetic, Local Rabbits, Sarcoptes scabiei, Scabies

Topic
Health and Medicine

Link: https://ifory.id/abstract/xebVvr2JHkgW

Corresponding Author
Pratiwi Soesilawati

Institutions
*Oral Biology Department, Faculty of Dental Medicine, Universitas Airlangga
Pedodontic Department, Faculty of Dental Medicine, Universitas Airlangga
Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga

Abstract
Dental implants have become a standard therapy to replace tooth loss. The healing process around implants is a complex process to explain whether the host can receive implant material and gain a perfect recovery process. In some cases these implants are unsuccessful because the infection or lack of oseointegration. This is called "early implant failure". Some things contribute to this situation such as surgical trauma, local and systemic factors, and genetic polymorphism factors. Increased levels of MMP-1 have been shown to occur in the sulcus fluid around implants that caused oseointegrated failure and periodontitis. The increase in MMP-1 titers is related to the degradation of collagen which has the most role as an oseointegrated molecular marker. The aim of this study was to develop a non-invasive method for genetic molecular diagnosis on dental implant patients through blood sampling at dental implant sockets. Method : the total 7 subjects, consisted of 4 men with an average age of 46.7 years and 3 women with an average age of 48.3 years. Genomic DNA examination was carried out through DNA extraction using the phenol-chloroform method. DNA amplification was carried out at the MMP-1 locus at 269 bp. The results of DNA isolation were examined for DNA purity and concentration, then used as a reference for determining the amount of DNA in the amplification of the MMP-1 locus by PCR usingMMP-1 primers. Electrophoresis was carried out using 3% agarose gel stained with EtBr. The electrophoresis results of all samples showed DNA band at 269 bp. This proves that DNA exploration in the dental implant socket is a non-invasive method, safe for patients and can be used in genomic analysis methods

Keywords
DNA exploration, dental implant, MMP-1, patiens

Topic
Health and Medicine

Link: https://ifory.id/abstract/XvEMaceUjw3y